NAD+ dosage calculator

500 mg + 5 mL = 100 mg/mL, so 50 mg is 0.5 mL, or 50 units on a U-100 syringe. Change the vial, the water or the amount and the syringe updates.

Also called: nicotinamide adenine dinucleotide, β-NAD, NAD+ dosage calculator, NAD+ reconstitution calculator.

NAD+ reconstitution calculator

Vial mg, water mL and amount give syringe units

mg

Total printed on the vial label.

mL

Diluent volume you add (1 mL = 100 units on a U-100 syringe).

Amount unit

The mass you want in one draw. 1 mg = 1,000 mcg.

Syringe barrel
Show U-40 and U-50 syringes

Result

50units

to draw on a U-100 syringe, 100-unit barrel

Vial: 500 mg with 5 mL diluent 500 mg +5 mL
Syringe filled to 50.0 of 100 units 0 10 20 30 40 50 60 70 80 90 100 U-100, 1 mL
Volume
0.5 mL
Concentration
100 mg/mL
Per unit
1,000 mcg
Draws per vial
10

500 mg + 5 mL = 100 mg/mL, so 50 mg is 0.5 mL, or 50 units on a U-100 syringe.

Arithmetic only. Not a dosing recommendation. Check every figure against your vial label and lab protocol.

How many units is each NAD+ amount?

At 500 mg + 5 mL (100 mg/mL) on a U-100 syringe:

Generated from the calculator's own functions. Rows are evenly spaced reference amounts, not suggested amounts. Amber readings don't fit a 100-unit barrel.

NAD+: 500 mg + 5 mL = 100 mg/mL, U-100 syringe
AmountmcgVolume (mL)Syringe units
10 mg 10,000 0.1 10
25 mg 25,000 0.25 25
50 mg 50,000 0.5 50
75 mg 75,000 0.75 75
100 mg 100,000 1 100
150 mg 150,000 1.5 150 (> 100)
200 mg 200,000 2 200 (> 100)
250 mg 250,000 2.5 250 (> 100)
Engraving of glass burettes, one with a dark strip behind its graduation to make the tick marks easier to read
A burette with a dark strip behind its graduation, 1895. Pick a water volume that lands your reading on a printed mark. Public domain.

How much water for each NAD+ vial size?

NAD+ is sold in 3 common sizes (100 mg, 500 mg, 1,000 mg). The same water volume gives a different concentration in each, so the same reading means a different amount. Each cell shows mcg per unit with mg/mL in brackets.

NAD+: mcg per U-100 unit (mg/mL in brackets) by vial size and water added
Vial2 mL5 mL10 mL
100 mg 500 (50)200 (20)100 (10)
500 mg 2,500 (250)1,000 (100)500 (50)
1,000 mg 5,000 (500)2,000 (200)1,000 (100)

How much bacteriostatic water for a 100 mg NAD+ vial?

2 mL → 50 mg/mL, so 50 mg is 100 units. 5 mL → 20 mg/mL, so 50 mg is 250 units. 10 mL → 10 mg/mL, so 50 mg is 500 units. The whole vial holds 2 draws of 50 mg.

How much bacteriostatic water for a 500 mg NAD+ vial?

2 mL → 250 mg/mL, so 50 mg is 20 units. 5 mL → 100 mg/mL, so 50 mg is 50 units. 10 mL → 50 mg/mL, so 50 mg is 100 units. The whole vial holds 10 draws of 50 mg.

How much bacteriostatic water for a 1,000 mg NAD+ vial?

2 mL → 500 mg/mL, so 50 mg is 10 units. 5 mL → 200 mg/mL, so 50 mg is 25 units. 10 mL → 100 mg/mL, so 50 mg is 50 units. The whole vial holds 20 draws of 50 mg.

Worked example: 500 mg vial, 5 mL, 50 mg

  1. Concentration. 500 mg ÷ 5 mL = 100 mg/mL (100,000 mcg/mL).
  2. Volume. 50 mg ÷ 100 mg/mL = 0.5 mL.
  3. Units. 0.5 mL × 100 = 50 units on a U-100 syringe.
  4. Check. One unit holds 1,000 mcg; 50 × 1,000 mcg = 50,000 mcg = 50 mg, which matches. The vial holds 10 draws of this size.

NAD+: notes for the arithmetic

NAD+ vials hold hundreds of milligrams, so amounts are in mg (not mcg) and diluent volumes are larger. A 500 mg vial needs 5 mL to reach 100 mg/mL. Check that your vial physically holds the volume you enter.

For the lab procedure itself (materials, aseptic technique, mixing without foaming) see how to reconstitute peptides. For what bacteriostatic water is and how long an opened vial is kept, see the bacteriostatic water guide and the storage guide.

Frequently asked questions

How many units is 50 mg of NAD+?

It depends on the water volume. 500 mg + 5 mL = 100 mg/mL, so 50 mg is 0.5 mL, or 50 units on a U-100 syringe. With 2 mL instead it would be 20 units.

How much bacteriostatic water for a 100 mg NAD+ vial?

There is no single correct volume. 2 mL gives 50 mg/mL (500 mcg per unit); 5 mL gives 20 mg/mL (200 mcg per unit); 10 mL gives 10 mg/mL (100 mcg per unit). Pick the volume that makes your reading easy to measure on the barrel you have.

How much bacteriostatic water for a 500 mg NAD+ vial?

There is no single correct volume. 2 mL gives 250 mg/mL (2,500 mcg per unit); 5 mL gives 100 mg/mL (1,000 mcg per unit); 10 mL gives 50 mg/mL (500 mcg per unit). Pick the volume that makes your reading easy to measure on the barrel you have.

How much bacteriostatic water for a 1,000 mg NAD+ vial?

There is no single correct volume. 2 mL gives 500 mg/mL (5,000 mcg per unit); 5 mL gives 200 mg/mL (2,000 mcg per unit); 10 mL gives 100 mg/mL (1,000 mcg per unit). Pick the volume that makes your reading easy to measure on the barrel you have.

How many mg of NAD+ is 10 units?

10 units on a U-100 syringe is 0.1 mL. At 100 mg/mL (500 mg in 5 mL) that is 10 mg (10,000 mcg). At a different concentration the answer is different.

Is this a NAD+ dosing calculator?

It is an arithmetic tool. It converts an amount you enter into a volume and a syringe reading; it does not suggest amounts, schedules or uses. NAD+ is a coenzyme, not a peptide. It is included because vials are reconstituted with the same arithmetic. Not approved by the FDA as an injectable drug.

Sources

  1. FDA alerts health care providers, compounders and patients of dosing errors associated with compounded injectable semaglutide products — U.S. Food and Drug Administration, 2024

Links checked 2026-10-19.