TB-500 dosage calculator

10 mg + 2 mL = 5 mg/mL, so 2 mg is 0.4 mL, or 40 units on a U-100 syringe. Change the vial, the water or the amount and the syringe updates.

Also called: thymosin beta-4 (fragment), Tβ4, TB-500 dosage calculator, TB-500 reconstitution calculator.

TB-500 reconstitution calculator

Vial mg, water mL and amount give syringe units

mg

Total printed on the vial label.

mL

Diluent volume you add (1 mL = 100 units on a U-100 syringe).

Amount unit

The mass you want in one draw. 1 mg = 1,000 mcg.

Syringe barrel
Show U-40 and U-50 syringes

Result

40units

to draw on a U-100 syringe, 100-unit barrel

Vial: 10 mg with 2 mL diluent 10 mg +2 mL
Syringe filled to 40.0 of 100 units 0 10 20 30 40 50 60 70 80 90 100 U-100, 1 mL
Volume
0.4 mL
Concentration
5 mg/mL
Per unit
50 mcg
Draws per vial
5

10 mg + 2 mL = 5 mg/mL, so 2 mg is 0.4 mL, or 40 units on a U-100 syringe.

Arithmetic only. Not a dosing recommendation. Check every figure against your vial label and lab protocol.

How many units is each TB-500 amount?

At 10 mg + 2 mL (5 mg/mL) on a U-100 syringe:

Generated from the calculator's own functions. Rows are evenly spaced reference amounts, not suggested amounts. Amber readings don't fit a 100-unit barrel.

TB-500: 10 mg + 2 mL = 5 mg/mL, U-100 syringe
AmountmcgVolume (mL)Syringe units
0.25 mg 250 0.05 5
0.5 mg 500 0.1 10
1 mg 1,000 0.2 20
1.5 mg 1,500 0.3 30
2 mg 2,000 0.4 40
2.5 mg 2,500 0.5 50
3 mg 3,000 0.6 60
5 mg 5,000 1 100
Engraving of glass burettes, one with a dark strip behind its graduation to make the tick marks easier to read
A burette with a dark strip behind its graduation, 1895. Pick a water volume that lands your reading on a printed mark. Public domain.

How much water for each TB-500 vial size?

TB-500 is sold in 2 common sizes (5 mg, 10 mg). The same water volume gives a different concentration in each, so the same reading means a different amount. Each cell shows mcg per unit with mg/mL in brackets.

TB-500: mcg per U-100 unit (mg/mL in brackets) by vial size and water added
Vial1 mL2 mL3 mL
5 mg 50 (5)25 (2.5)16.67 (1.67)
10 mg 100 (10)50 (5)33.33 (3.33)

How much bacteriostatic water for a 5 mg TB-500 vial?

1 mL → 5 mg/mL, so 2 mg is 40 units. 2 mL → 2.5 mg/mL, so 2 mg is 80 units. 3 mL → 1.67 mg/mL, so 2 mg is 120 units. The whole vial holds 2 draws of 2 mg.

How much bacteriostatic water for a 10 mg TB-500 vial?

1 mL → 10 mg/mL, so 2 mg is 20 units. 2 mL → 5 mg/mL, so 2 mg is 40 units. 3 mL → 3.33 mg/mL, so 2 mg is 60 units. The whole vial holds 5 draws of 2 mg.

Worked example: 10 mg vial, 2 mL, 2 mg

  1. Concentration. 10 mg ÷ 2 mL = 5 mg/mL (5,000 mcg/mL).
  2. Volume. 2 mg ÷ 5 mg/mL = 0.4 mL.
  3. Units. 0.4 mL × 100 = 40 units on a U-100 syringe.
  4. Check. One unit holds 50 mcg; 40 × 50 mcg = 2,000 mcg = 2 mg, which matches. The vial holds 5 draws of this size.

TB-500: notes for the arithmetic

Products sold as "TB-500" are not consistent: some labels describe full-length thymosin beta-4 and some a short acetylated fragment (Ac-LKKTETQ). The arithmetic is the same either way because it works in mg, but compare like with like.

For the lab procedure itself (materials, aseptic technique, mixing without foaming) see how to reconstitute peptides. For what bacteriostatic water is and how long an opened vial is kept, see the bacteriostatic water guide and the storage guide.

Frequently asked questions

How many units is 2 mg of TB-500?

It depends on the water volume. 10 mg + 2 mL = 5 mg/mL, so 2 mg is 0.4 mL, or 40 units on a U-100 syringe. With 1 mL instead it would be 20 units.

How much bacteriostatic water for a 5 mg TB-500 vial?

There is no single correct volume. 1 mL gives 5 mg/mL (50 mcg per unit); 2 mL gives 2.5 mg/mL (25 mcg per unit); 3 mL gives 1.67 mg/mL (16.67 mcg per unit). Pick the volume that makes your reading easy to measure on the barrel you have.

How much bacteriostatic water for a 10 mg TB-500 vial?

There is no single correct volume. 1 mL gives 10 mg/mL (100 mcg per unit); 2 mL gives 5 mg/mL (50 mcg per unit); 3 mL gives 3.33 mg/mL (33.33 mcg per unit). Pick the volume that makes your reading easy to measure on the barrel you have.

How many mg of TB-500 is 10 units?

10 units on a U-100 syringe is 0.1 mL. At 5 mg/mL (10 mg in 2 mL) that is 0.5 mg (500 mcg). At a different concentration the answer is different.

Is this a TB-500 dosing calculator?

It is an arithmetic tool. It converts an amount you enter into a volume and a syringe reading; it does not suggest amounts, schedules or uses. Not approved by the FDA for any use. Sold as a research chemical only.

Sources

  1. FDA alerts health care providers, compounders and patients of dosing errors associated with compounded injectable semaglutide products — U.S. Food and Drug Administration, 2024

Links checked 2026-10-19.